1. Restriction enzymes are known as molecular scissors, because it is used to cut specific sequences at specific site.
2. It is widely used in molecular labs. It is classified under endonuclease enzyme means it cuts more than 3 - 4 sequences.
3. It is divided into 3 types, type 1 RE, type 2 RE and type 3 RE.
4. Type 2 is most widely used enzyme, because it cuts more than 9 - 10bp at specific site. It requires ATP, NADP and acetyl coA as its co-factor.