in Principles and process of Biotechnology by

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Plasmids are autonomously replicating circular extra chromosomal DNA. Plasmids are small circular piece of DNA that are found naturally in some bacterial cells in addition to the main bacterial chromosome.

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for PMT preparation, these following things can be asked atmost,

small circular

double stranded,

naked(no histone association),

extrachromosomal DNA having useful but Non-Vital genes eg; fertility F factor, Resistance R factor

can replicate independently

when they are attacehed to nucleoid or genophore this association is called EPISOME

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1. Plasmids are the extrachromosomal DNA present in the nucleus.

2. They are self replicating, hence it can be used in many biotechnological process.

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