Login
Remember
Register
Topic Wise Biology MCQs
All Activity
Q&A
Questions
Hot!
Unanswered
Tags
Categories
Ask a Question
Ask a Question
Home
Biotechnology
what are the limitations PCR
what are the limitations PCR
asked
in
Biotechnology
by
owusu-fordjour collins
application-of-biotechnology
Your comment on this question:
Your name to display (optional):
Email me at this address if a comment is added after mine:
Email me if a comment is added after mine
Privacy: Your email address will only be used for sending these notifications.
Anti-spam verification:
To avoid this verification in future, please
log in
or
register
.
Your answer
Your name to display (optional):
Email me at this address if my answer is selected or commented on:
Email me if my answer is selected or commented on
Privacy: Your email address will only be used for sending these notifications.
Anti-spam verification:
To avoid this verification in future, please
log in
or
register
.
1
Answer
0
votes
answered
by
Lifeeasy Authors
Use of PCR requires prior knowledge of at least a part of the target DNA sequence for the construction of primers.
Taq polymerase lacks proofreading ability. It incorporates an incorrect nucleotide about once every 20,000bp, thus affecting accuracy.
Capacity of PCR to amplify extremely small amounts of DNA makes contamination a significant problem.
Taq polymerase can only amplify DNA fragments less than 2000bp in size.
Your comment on this answer:
Your name to display (optional):
Email me at this address if a comment is added after mine:
Email me if a comment is added after mine
Privacy: Your email address will only be used for sending these notifications.
Anti-spam verification:
To avoid this verification in future, please
log in
or
register
.
← Prev Question
Next Question →
Related questions
1
answer
What are the requirements for PCR (Polymerase Chain Reaction)?
asked
in
Biotechnology and its Applications
by
Lifeeasy Biology
application-of-biotechnology
polymerase
1
answer
What are the applications of PCR in biotechnology?
asked
in
Biotechnology and its Applications
by
Lifeeasy Biology
application-of-biotechnology
1
answer
Steps involved in PCR (Polymerase Chain Reaction) process.
asked
in
Biotechnology and its Applications
by
Lifeeasy Biology
application-of-biotechnology
polymerase
1
answer
What are the applications of cDNA library?
asked
in
Biotechnology and its Applications
by
Lifeeasy Biology
application-of-biotechnology
1
answer
What are the restriction fragments?
asked
in
Biotechnology and its Applications
by
Lifeeasy Biology
application-of-biotechnology
Categories
All categories
Diversity in Living World - Kingdoms
(658)
Structural Organisation in Animals and Plants
(438)
Cell Structure and Function
(552)
Plant Physiology
(705)
Human Physiology
(443)
Genetics and Evolution
(369)
Biology and Human Welfare
(249)
Biotechnology
(247)
Biotechnology and its Applications
(87)
Principles and process of Biotechnology
(147)
Application of Biotechnology in health and agriculture
(7)
Health and Disease
(156)
Ecology and Environment
(109)
Reproduction
(336)
...