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  1. Use of PCR requires prior knowledge of at least a part of the target DNA sequence for the construction of primers.
  2. Taq polymerase lacks proofreading ability. It incorporates an incorrect nucleotide about once every 20,000bp, thus affecting accuracy.
  3. Capacity of PCR to amplify extremely small amounts of DNA makes contamination a significant problem.
  4. Taq polymerase can only amplify DNA fragments less than 2000bp in size.
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