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The main applications of the protoplasmic isolation are:

  1. By protoplasmic isolation and its subsequent fusion of hybrids of distant species of plants can be brought together.
  2. The isolated protoplasm is used for selection of mutant lines, cloning and also for studying the protoplasm.
  3. It is also for studying the biosynthesis of cell wall.
  4. The DNA ingestion can be done there by the cell can be modified.
  5. The regeneration of the plants from the callus which is produced as a result of the protoplast culture.