The main requirements for the formation of the somatic embryos are:
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The amount of the auxin concentration should be high for the embryo induction.
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The auxin concentration is reduced for the growth and development of the embryo.
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The presence of the phyto hormones gibberellins and the ethylene inhibits the somatic embryogenesis.
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Temperature, light, extracts (Coconut water) etc. favours the embryogenesis.
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Presence of nitrogen is another important factor needed for initiation of embryogenesis.
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The callus from the young part of the plants is usually more efficient.
