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  1. The first step in PCR is denaturation of DNA which is carried out at high temperatures of about 94oC.
  2. Taq polymerase is a heat-stable (thermostable) enzyme isolated from the bacterium Thermus aquaticus which is a thermophilic (heat-loving) bacterium.
  3. Taq polymerase does not get denatured at high temperature and is capable of withstanding high temperatures up to 95oC.

Hence, the enzyme Taq polymerase is used in Polymerase Chain Reaction.

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