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Polymerase chain reaction is used for the amplification of DNA molecules. PCR is important as it is widely used in:

  1. Clinical diagnosis.
  2. Prenatal diagnosis of inherited diseases.
  3. Diagnosis of retroviral infections.
  4. Diagnosis of bacterial infections.
  5. Diagnosis of cancers.
  6. Sex determination of embryos.
  7. DNA sequencing.
  8. Gene manipulation and expression studies.
  9. Comparative studies of genomes.
  10.  Forensic sciences.
  11. Comparison with gene cloning.
  12. DNA based phylogeny
  13. Functional analysis of a gene.
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