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Difference between DNA polymerase i and iii

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No.

Feature

DNA Polymerase I

DNA Polymerase III

1.

Polymerization 5’→3’

Yes

Yes

2.

Exonuclease 3’→’5

Yes

Yes

3.

Exonuclease 5’→3’

Yes

No

4.

Use of primer single strands

Yes

No

5.

Use of nicked duplex or helix

Yes

No

6.

Molecular weight

109000

(single chain)

>250000

(heteromultimeric chain)

7.

Polypeptide chains

Two

Mol. Wt.

Large - 75,000

Small – 36,000

Two

Mol. Wt.

Large - 1,40,000

Small – 40,000

8.

No. of molecules per cell

400

10- 20

9.

Genes

pol A

pol C (dna E), dna N, dna ZX, dna Q, dna T.

10.

No. of nucleotides polymerized at 37oC molecules/ minute

Up to 1000

Up to 15000

11.

Affinity for nucleoside triphosphate (NTPs) precursors

Low

High

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