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GLYCEROL ACTS AS DNA ATTACHMENT SUBSTANCE.... AND ALSO INCREASE  CELL POROSITY TO MAKE THE DNA TO ENTER INSIDE THE CELL EASILY....  PEG  I.E. POLY ETHYLENE GLYCO  USE  DURING CELL TRANSFORMATION  TO INCREASE THE TRANSFORMATION EFFICIENCY....
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It is a cryoprotectant with a higher freezing point than water. It protects the bacterial cells from the formation of ice crystals during -80 C freezing and storage. Glycerol excludes water from the space between cells and facilitates quicker freezing with its higher freezing point.
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