in Biotechnology and its Applications by

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Denaturation is the process of chemical and physical breakdown of hydrogen bonds which results in deformation of specific structure and loss of its function.

EXAMPLE: Denaturation of DNA breaks the hydrogen bonds between their base pairs, results in the release of nucleic acids.

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It is the first step involved in PCR. It refers to breakdown of hydrogen bonds between the base pairs at a temperature of 90 - 94°C. It results in single stranded DNA.
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