in Biotechnology and its Applications by

1 Answer

0 votes
  1. The first step in PCR is denaturation of DNA which is carried out at high temperatures of about 94oC.
  2. Taq polymerase is a heat-stable (thermostable) enzyme isolated from the bacterium Thermus aquaticus which is a thermophilic (heat-loving) bacterium.
  3. Taq polymerase does not get denatured at high temperature and is capable of withstanding high temperatures up to 95oC.

Hence, the enzyme Taq polymerase is used in Polymerase Chain Reaction.

Biology Questions and Answers for Grade 10, Grade 11 and Grade 12 students, Junior and Senior High Schools, Junior Colleges, Undergraduate biology programs and Medical Entrance exams.