in Principles and process of Biotechnology by

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The two types of ‘biological tools’ used in recombinant DNA technology are:

  1. Enzymes: These include Restriction enzymes (Endonucleases), which acts as molecular scissors, Ligases which are responsible for joining DNA fragments together and Reverse Transcriptase which is used for converting mRNA to cDNA.
  2. Vectors: They serve as a medium or vehicle to carry recombinant DNA into the host cell. The most commonly used vectors as Plasmids, Cosmids and Bacteriophages.
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