in Principles and process of Biotechnology by

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The steps involved in rDNA technology are:

  1. Isolation of DNA
  2. Fragmentation of the DNA using the enzyme Restriction endonucleases
  3. Isolation of the desired DNA fragment
  4. Amplification of the gene of interest
  5. Ligation of the DNA fragment into a suitable vector by the enzyme DNA ligases
  6. Transfer of DNA into the host cell
  7. Culturing the host cells on a suitable medium on a large scale
  8. Extraction of the desired product
  9. Downstream processing of the products
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